Antimicrobial activity and phytochemical screening of secondary metabolites extracted from the ascidian rhopalaea birkelandi artículo Académico uri icon


  • Samples of R. birkelandi were collected, milled, pulverized, air dried, lyophilized and subjected to gradient extraction with ethyl acetate, acetone, ethanol and methanol to purify its secondary metabolites. Extracts were screened phytochemically for its chemical components. In vitro antibacterial assay of the extracts, by the use of agar-well diffusion method, revealed that the extracts have broad spectrum activity on S. aureus strain. The ethyl acetate and acetone extracts exhibited antimicrobial activity with inhibition zones of 7.00 and 22.00 mm respectively. No activity was observed in the ethanol and methanol extract. The minimum inhibitory concentration (MIC) of the ethyl acetate extract was 10.00 mg/mL, while that of acetone extract was 2.50 mg/mL. The minimum bactericidal concentration (MBC) for ethyl acetate was 10.00 mg/mL, while the acetonic one was 2.50 mg/mL. Phytochemical screening revealed the presence of reducing compounds, terpenes, steroids, and amino acids in the extracts. The activities observed could be attributed to the presence of some of the detected phytochemical substances which have been formerly associated with antibacterial activity. The ability of the crude extracts of R. birkelandi to inhibit the growth of bacteria is an indication of its broad potential antimicrobial spectrum, and therefore it could be employed in the management of microbial infections. © EuroJournals Publishing, Inc. 2012.

fecha de publicación

  • 2012